Download PDF by M. Cristina Vega: Advanced Technologies for Protein Complex Production and

By M. Cristina Vega

ISBN-10: 3319272144

ISBN-13: 9783319272146

This publication offers complicated expression applied sciences for the construction of protein complexes. when you consider that complexes lie on the center of recent biology, the expression, purification, and characterization of huge quantities of top of the range protein complexes is important for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to advanced reconstitution from person subunits, this ebook bargains worthy insights and counsel for winning protein expressionists.

Across numerous sections readers will become aware of present possibilities for the creation of protein complexes in bacterial platforms (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary procedure. a final part introduces in brief a number of the biophysical and structural characterization suggestions for macromolecular complexes utilizing state of the art resolution scattering and nuclear magnetic resonance.

This paintings is a guided journey over probably the most robust and profitable protein expression applied sciences, with a spotlight on co-expression and high-throughput functions. it's addressed to each person drawn to the creation and characterization of macromolecular complexes, from collage scholars who wish an obtainable description of the main co-expression platforms to researchers in biomedicine and the lifestyles sciences looking for an up to date survey of accessible technologies.

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Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega

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